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TE-8214 binds to HSA and SSTR2 and retains octreotide-like potency. SPR sensorgrams (response vs. time) showing concentration-dependent association/dissociation phases of TE-8214 with HSA (A) or SSTR2 (B) ; fits to a 1:1 Langmuir model yielded dissociation constants (KD) of 1.86 mM forHSA-TE-8214 and 0.80 mM for SSTR2-TE-8214. Dose-dependent reduction of intracellular cAMP levels in QGP-1 (C) and <t>AR42J</t> (D) cells after a 20-minute incubation with TE-8214 or octreotide. Dose-dependent cell viability reductions in QGP-1 (E) and AR42J (F) cells after a 3-day incubation with TE-8214 or octreotide. Data are mean ± SD; n = 3.
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TE-8214 binds to HSA and SSTR2 and retains octreotide-like potency. SPR sensorgrams (response vs. time) showing concentration-dependent association/dissociation phases of TE-8214 with HSA (A) or SSTR2 (B) ; fits to a 1:1 Langmuir model yielded dissociation constants (KD) of 1.86 mM forHSA-TE-8214 and 0.80 mM for SSTR2-TE-8214. Dose-dependent reduction of intracellular cAMP levels in QGP-1 (C) and AR42J (D) cells after a 20-minute incubation with TE-8214 or octreotide. Dose-dependent cell viability reductions in QGP-1 (E) and AR42J (F) cells after a 3-day incubation with TE-8214 or octreotide. Data are mean ± SD; n = 3.

Journal: Frontiers in Endocrinology

Article Title: A long-acting octreotide analog formed by conjugation with a peptide linker modified with two fatty acids

doi: 10.3389/fendo.2026.1871977

Figure Lengend Snippet: TE-8214 binds to HSA and SSTR2 and retains octreotide-like potency. SPR sensorgrams (response vs. time) showing concentration-dependent association/dissociation phases of TE-8214 with HSA (A) or SSTR2 (B) ; fits to a 1:1 Langmuir model yielded dissociation constants (KD) of 1.86 mM forHSA-TE-8214 and 0.80 mM for SSTR2-TE-8214. Dose-dependent reduction of intracellular cAMP levels in QGP-1 (C) and AR42J (D) cells after a 20-minute incubation with TE-8214 or octreotide. Dose-dependent cell viability reductions in QGP-1 (E) and AR42J (F) cells after a 3-day incubation with TE-8214 or octreotide. Data are mean ± SD; n = 3.

Article Snippet: Intracellular CgA levels in AR42J cell lysates (diluted 1:5 with Standard/Sample Diluent) was quantified by Rat CgA ELISA (RK03567, ABclonal) by measuring optical density at 450 nm.

Techniques: Concentration Assay, Incubation